design optimization™ Search Results


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Transnetyx pcr genotyping
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PharmaSci Consulting targeted prodrug design to optimize drug delivery
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Shanghai GenePharma optimized crispr design application
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Optimized Crispr Design Application, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/optimized crispr design application/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
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SAS institute d-efficient fractional factorial design
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
D Efficient Fractional Factorial Design, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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ANSYS inc finite element analysis and the optimization design of the yj3128-type dump truck’s sub-frames
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Finite Element Analysis And The Optimization Design Of The Yj3128 Type Dump Truck’s Sub Frames, supplied by ANSYS inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/finite element analysis and the optimization design of the yj3128-type dump truck’s sub-frames/product/ANSYS inc
Average 90 stars, based on 1 article reviews
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ANSYS inc initial model optimization design
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Initial Model Optimization Design, supplied by ANSYS inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/initial model optimization design/product/ANSYS inc
Average 90 stars, based on 1 article reviews
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PetroChina Co Ltd frsmart integrated geo-engineering fracturing design software
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Frsmart Integrated Geo Engineering Fracturing Design Software, supplied by PetroChina Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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PrimerDesign Inc optimal primer design rules
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Optimal Primer Design Rules, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Oncoimmunity commercially available advanced software to predict optimal peptides for vaccine design
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Commercially Available Advanced Software To Predict Optimal Peptides For Vaccine Design, supplied by Oncoimmunity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Verlag GmbH optimal shape design for elliptic systems
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Optimal Shape Design For Elliptic Systems, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Verlag GmbH stochastic distribution control systems design: a convex optimization approach
The stable SH-SY5Y cells, <t>expressing</t> <t>AMPKα1</t> shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing <t>CRISPR/Cas-9-AMPKα1</t> (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.
Stochastic Distribution Control Systems Design: A Convex Optimization Approach, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The stable SH-SY5Y cells, expressing AMPKα1 shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing CRISPR/Cas-9-AMPKα1 (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.

Journal: Oncotarget

Article Title: AMPK activation by Tanshinone IIA protects neuronal cells from oxygen-glucose deprivation

doi: 10.18632/oncotarget.23391

Figure Lengend Snippet: The stable SH-SY5Y cells, expressing AMPKα1 shRNA (“S1/2”), or scramble control shRNA (“shC”) ( A – D ) as well as expressing CRISPR/Cas-9-AMPKα1 (“AMPKα1-KO”) or CRISPR/Cas-9 control (“CRISPR-C”), were pre-treated with 10 μM of Tanshinone IIA (“ThIIA”), with or without oxygen glucose deprivation (OGD, for 4 hours) and re-oxygenation (ODGR); AMPKα1 mRNA level was tested by qRT-PCR assay (A and E ); Listed proteins in total cell lysates were shown (B and F ); Cell survival and cell death were tested by MTT assay (C and G ) and LDH release assay (D and H ), respectively. Data were presented as mean ± SD ( n = 5). * P < 0.05 vs. “Mock” group. # P < 0.05 vs. “shC” cells (C–D) or “CRISPR-C” cells (G and H). Experiments in this figure were repeated three times, and similar results were obtained.

Article Snippet: The small guide RNA (sgRNA) targeting human AMPKα1 was based on the Optimized CRISPR Design application ( http://crispr.mit.edu/ ), which was provided by Genepharm (Shanghai, China).

Techniques: Expressing, shRNA, Control, CRISPR, Quantitative RT-PCR, MTT Assay, Lactate Dehydrogenase Assay